AKTIVE PROSJEKTER

 

Hvordan påvirker proteinmodifikasjoner hjernen vår?

Et av proteinene som jeg karakteriserte i løpet av doktorgraden i Arnesen lab var relativt lite studert da jeg startet. Det ble tydelig at dette enzymet hadde en rolle i cellenes sekretoriske system og i tillegg var det noen vage antydninger på at det kunne være svært viktig for nerveceller, noe jeg fattet spesiell interesse for siden jeg har en bakgrunn i molekylær nevrobiologi fra Bramham lab. Gjennom et nytt samarbeid med nevrologer på UCL holder jeg nå på å avdekke klare beviser for at dette proteinet har en kritisk funksjon i hjernen. For tiden studerer jeg celler som er isolert fra pasienter. Disse pasientene er rammet av arvelig nevrologisk sykdom fordi de mangler en bestemt type proteinmodifikasjon. Dette er utrolig spennende fordi vi er inne på et spor hvor det kan bli mulig å beskrive hvordan molekylære mekanismer forårsaket av et protein inni hjernecellene har en avgjørende nevrobiologisk betydning. Det er grunnlag for å tro at det kan være en kobling mellom NAT enzymer og nevrodegenerative sykdommer som Parkinsons. Dette er noe jeg brenner for å studere videre. Forekomsten av nevrodegenerative sykdommer øker, mens behandlingen har store utfordringer. Det er derfor et trykkende behov for forskning på dette området. Jeg håper at jeg gjennom kombinert spesialkunnskap om NAT enzymfamilien og molekylær nevrobiologi vil kunne bidra til å fylle dette kunnskapsgapet.

 

 

Kan mangel på aktin N-terminal modifikasjon i kreft være knyttet til økt risiko for metastase?

Iløpet av postdoktor perioden var jeg med på å karakterisere et enzym, kalt NAA80, som modifiserer aktins N-terminal. Aktin er et av de proteinene det finnes mest av i cellene våre og det er byggesteinen i mikrofilament celleskjelett. Dette celleskjelettet er ikke stivt og statisk sånn som navnet kanskje skulle tilsi, men er ekstremt dynamisk og denne konstante endringen er bl.a. grunnlaget for cellers bevegelighet. Normalt sett i en ferdig utviklet organisme er cellenes bevegelighet holdt i sjakk slik at en levercelle holder posisjonen sin i leveren og en nyrecelle holder posisjonen sin i nyren. Men i kreft kan reguleringen være forstyrret og det kan medføre at kreftceller begynner å invadere nabovev og migrere til andre steder i kroppen, såkalt metastase. Denne prosessen fattet jeg spesielt interesse for da vi oppdaget at når vi fjernet NAA80 enzymet, så ble cellene hypermobile. Videre har jeg nå sammen med studenter fintunet verktøy for å studere denne prosessen, samt beskrevet videre at Golgi-apparatet er fragmentert i celler som mangler NAA80.

I 2022 er jeg medarrangør og foredragsholder på en stor internalsjonal vitenskapelig konferanse i Bergen 8.-11. Juni. Se vårt spennende program: EMBO conference "Protein termini: from mechanisms to biological impact". 

 

Henriettes forskningsformidling og medieomtale

• Fjerning av protein N-terminal acetylering gir degradering av ribosomale proteiner. UiB populærvitenskapelig artikkel tilhørende vitenskapelig publikasjon.
• Tildelt Meltzer prisen for fremragende yngre forskere 2022. UiB annonsering og intervju. 
• Diving into the microscopic world. Sci Snapshot forskerportrett i NatureVolve Magazine.
• Gjær og proteomikk definerer protein N-terminal acetylering. UiB populærvitenskapelig artikkel tilhørende vitenskapelig publikasjon.
• Poster pris til Alessia Caiella basert på vårt arbeid, EMBO/ISPT konferansen 2022.
• Feil ved nytt gen årsak til utviklingshemming. UiB populærvitenskapelig artikkel tilhørende vitenskapelig publikasjon.
• Application note, nytt bruksområde for det digtale holografiske mikroskopet HoloMonitor M4, basert på vårt arbeid.
• Horizon Discovery blog skriver om Hvordan lykkes med HAP1 celler, basert på vårt arbeid.
• First-person interview i Biology Open med studentene Tobias B. Beigl, Ine Kjosås and Emilie Seljeseth basert på vårt arbeid.
• Fra prosjektoppgave i MOL231 til publikasjon, UiB nyhetsartikkel av studentene Ine Kjosås og Emilie Seljeseth.
• Vinner av Falch junior award 2019, UiB annonsering, UiB artikkel intervju.
• Å forske fra hjemmekontor, UiB artikkel om arbeid sammen med MSc studenter Monica Hellsevik og Hanne Øye.
• Fra ERASMUS utveksling til publisert artikkel, UiB artikkel om arbeid sammen med MSc student Tobias B. Beigl.
• A Jack of all Trades in Cancer Cells, UiB artikkel i anledning review i MolCel 2019.
• Avdekker hemmelighetene bak cellers bevegelighet, InCyte blog intervju.
• Hyper-bevegelige celler avslørt med IncuCyte, InCytes Publication newsletter. 
• Setter brems på cellebevegelighet, UiB artikkel.
• Masteravhandlingen ble TIRFet i land (som hovedveileder for Monica Hellesvik), UiB artikkel
• Trener forskingsformidling foran kamera i Momentum karriereprogram for fremragende yngre forskere, UiB artikkel
• Fakultetets dag Medisinsk fakultet  - Mottok på vegne av alle medforfattere i Arnesen lab, pris for beste publikasjon i 2018 for back-to-back artikler i PNAS 2018 UiB artikkel, MedFak og UiB artikkel, IBM
• Cellespionasje i NAT laboratoriet, UiB artikkel
• Protein acetylation gets some respect—A commentary by Henriette Aksnes, Cell Reports blog
• Actin acetylation, NBS Nytt 2 2018
• Protein function can be revealed by microscopy, UiB artikkel
• Hangin’ out at the Golgi, UiB artikkel
• Cellular GPS-system reveals new territory for an influential enzyme family, UiB artikkel
• ZONA award for beste PhD 2014, UiB artikkel 
• Ny doktorgrad PhD, UiB pressemelding

Kurs

BMED320 - Metoder i Medisinsk Cellebiologi

                 Forskning- og laboratorieopplæring for prosjektoppgaver på Master nivå.

                 Gjesteforelseser i tema Mikroskopi.

MOL270 - Bioetikk

               Gjesteforeleser i tema Modelorganismer.

MOL231 - Prosjektoppgave i Molekylærbiologi

               Forskning- og laboratorieopplæring for prosjektoppgaver på Bachelor nivå.

 

Studentveiledning

2024-         Hovedveileder for MSc stud., Dept. of Biological sciences, UiB. Anton R. Melbye

2023-         Hovedveileder for forskerlinjestudent, MED, UiB. Christina W. Leerink

2023-24     Hovedveileder for Masterstud., Dept. of Biomedicine, UiB. Åse K. Bekkelund

2023-         Hovedveileder for PhD candidate, Dept. of Biomedicine, UiB. Alessia Caiella

2023-         Medveileder for PhD candidate, Dept. of Biomedicine, UiB. Silje K. Larsen

2022-23    Hovedveileder for Masterstud., Dept. of Biomedicine, UiB. Anette Siggervåg

2022-23    Medveileder for Masterstud., Dept. of Biomedicine, UiB. Inger Johanne Hellerud

2021–22     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Therese S. Hjellvoll

2021–22     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Ajia R. Pennavaria

2021–22     Co-supervisor for MSc stud., Dept. of Biological sciences, UiB. Camilla H. Nundal

2021-22      Co-supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Birger H. Furnes 

2021–         Main supervisor for PhD candidate, Dept. of Biomedicine, UiB. Hanne Øye

2020–         Co-supervisor for PhD candidate, Dept. of Biomedicine, UiB. Monica Hellesvik

2020–21     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Alessia Caiella

2020–21     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Liv S. Krogstad

2020–21     Co-supervisor for MSc thesis stud., Dept. of Biological sciences, UiB. Ine Kjosås

2019–20     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Kristine H. Furre

2019–20     Main supervisor for MSc thesis stud., Dept. of Biomedicine, UiB. Hanne Øye

2018–19     Main supervisor for MSc thesis stud., Dept. of Biological Sciences, UiB, Monica Hellesvik

2019–19     Main supervisor for MSc thesis stud., UiB/Universität Stuttgart, Tobias B. Beigl

2019–19     Main supervisor for 2 BSc students in MOL231 project course, Dept. of Biological Sciences, UiB, Ine Kjosås and Emilie Seljeseth

2018–18     Main supervisor for 2 MSc students in BMED320 project course, Dept. of Biomedicine, UiB. Atefeh Kianian and Kristine H. Furre

2018–18     Main supervisor for MSc ERASMUS stud., Dept. of Biomedicine, UiB, Tobias B. Beigl

2014–17     Co-supervisor for PhD candidate, Dept. of Molecular biology, UiB, Marianne Goris

2013–16     Co-supervisor for PhD candidate, Dept. of Molecular biology, UiB, Sylvia Varland

2012–16     Co-supervisor for PhD candidate, Dept. of Molecular biology, UiB, Camilla Osberg

2011–12     Co-supervisor for MSc thesis stud., Dept. of Molecular biology, UiB, Camilla Osberg

2011–12     Co-supervisor for MSc thesis stud., Dept. of Molecular biology, UiB, Sylvia Varland

2011–12     Co-supervisor for BSc stud., Dept. of Molecular biology, UiB, Eilen Henriksen

Vitenskapelig artikkel

• Chelban, Viorica; Aksnes, Henriette; Maroofian, Reza et al. (2024). Biallelic NAA60 variants with impaired n-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications. (ekstern lenke)
• Larsen, Silje Kathrine; Bekkelund, Åse Karine; Glomnes, Nina et al. (2024). Assessing N-terminal acetylation status of cellular proteins via an antibody specific for acetylated methionine. (ekstern lenke)
• Guzman, Ulises H.; Aksnes, Henriette; Ree, Rasmus et al. (2023). Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae. (ekstern lenke)
• Aksnes, Henriette; McTiernan, Nina ; Arnesen, Thomas (2023). NATs at a glance. (ekstern lenke)
• Van Damme, Petra; Osberg, Camilla; Jonckheere, Veronique et al. (2022). Expanded in vivo substrate profile of the yeast N-terminal acetyltransferase NatC. (ekstern lenke)
• Beigl, Tobias; Kjosås, Ine; Seljeseth, Emilie et al. (2020). Efficient and crucial quality control of HAP1 cell ploidy status. (ekstern lenke)
• Beigl, Tobias; Hellesvik, Monica; Saraste, Jaakko et al. (2020). N-terminal acetylation of actin by NAA80 is essential for structural integrity of the Golgi apparatus. (ekstern lenke)
• Hellesvik, Monica; Øye, Hanne; Aksnes, Henriette (2020). Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays. (ekstern lenke)
• Varland, Sylvia; Aksnes, Henriette; Kryuchkov, Fedor et al. (2018). N-terminal acetylation levels are maintained during acetyl-CoA deficiency in Saccharomyces cerevisiae. (ekstern lenke)
• Aksnes, Henriette; Marie, Michaël Bruno Eric; Arnesen, Thomas et al. (2018). Actin polymerization and cell motility are affected by NAA80-mediated posttranslational N-terminal acetylation of actin. (ekstern lenke)
• Drazic, Adrian; Aksnes, Henriette; Marie, Michael Bruno Eric et al. (2018). NAA80 is actin’s N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. (ekstern lenke)
• Aksnes, Henriette; Goris, Marianne; Strømland, Øyvind et al. (2017). Molecular determinants of the N-Terminal acetyltransferase Naa60 anchoring to the Golgi membrane. (ekstern lenke)
• Osberg, Camilla; Aksnes, Henriette; Ninzima, Sandra et al. (2016). Microscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases. (ekstern lenke)
• Aksnes, Henriette; Hole, Kristine; Arnesen, Thomas (2015). Molecular, cellular, and physiological significance of N-terminal acetylation. (ekstern lenke)
• Aksnes, Henriette; Van Damme, Petra; Goris, Marianne et al. (2015). An organellar Nα-acetyltransferase, Naa60, acetylates cytosolic n termini of transmembrane proteins and maintains golgi integrity. (ekstern lenke)
• Aksnes, Henriette; Osberg, Camilla; Arnesen, Thomas (2013). N-Terminal Acetylation by NatC Is Not a General Determinant for Substrate Subcellular Localization in Saccharomyces cerevisiae. (ekstern lenke)
• Hole, Kristine; Van Damme, Petra; Dalva, Monica et al. (2011). The human N-Alpha-Acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4. (ekstern lenke)

Vitenskapelig oversiktsartikkel/review

• Arnesen, Thomas; Aksnes, Henriette; Giglione, Carmela (2023). Protein Termini 2022: central roles of protein ends. (ekstern lenke)
• Arnesen, Thomas; Aksnes, Henriette (2023). Actin finally matures: uncovering machinery and impact. (ekstern lenke)
• Aksnes, Henriette; Ree, Rasmus Moen; Arnesen, Thomas (2019). Co-translational, post-translational, and non-catalytic roles of N-terminal acetyltransferases. (ekstern lenke)
• Aksnes, Henriette; Drazic, Adrian; Marie, Michael Bruno Eric et al. (2016). First things first: Vital protein marks by N-terminal acetyltransferases. (ekstern lenke)
• Aksnes, Henriette; Drazic, Adrian; Arnesen, Thomas (2015). (Hyper)tension release by N-terminal acetylation. (ekstern lenke)

Mastergradsoppgave

• Siggervåg, Anette; Aksnes, Henriette; Arnesen, Thomas (2023). NAA60 in neurodegenerative disease. (ekstern lenke)
• Hellerud, Inger Johanne; Arnesen, Thomas; Aksnes, Henriette (2023). Investigating the biological role of human NAT14 (probable N-acetyltransferase 14). (ekstern lenke)
• Furnes, Birger Hjønnevåg; Arnesen, Thomas; Hellesvik, Monica et al. (2022). The mechanisms by which actin Nt-acetylation steers cell migration. (ekstern lenke)
• Pennavaria, Ajia Rae; Aksnes, Henriette; Arnesen, Thomas (2022). Molecular roles of NAA60 in neurodegeneration. (ekstern lenke)
• Hjellvoll, Therese Soltvedt; Aksnes, Henriette; Arnesen, Thomas (2022). Defining properties of α-synuclein using human NatB knockout cells. (ekstern lenke)
• Krogstad, Liv Svenningsson; Aksnes, Henriette; Arnesen, Thomas (2021). Uncovering NAA60’s Role in the Nervous System. (ekstern lenke)
• Caiella, Alessia; Aksnes, Henriette; Arnesen, Thomas (2021). The effect of N-terminal acetylation on α-Synuclein’s pathogenicity in Parkinson’s disease. (ekstern lenke)
• Furre, Kristine Haugen; Aksnes, Henriette; Arnesen, Thomas (2020). The Impact of N-terminal Acetylation on Actin Polymerization and Cell Migration. (ekstern lenke)
• Øye, Hanne; Aksnes, Henriette; Arnesen, Thomas (2020). Impact of Actin N-terminal Acetylation on Small Cell Lung Carcinoma Cell Invasiveness. (ekstern lenke)
• Bramham, Clive R. Evjen; Aksnes, Henriette (2009). Time-lapse imaging of Arc/Arg3.1 protein degradation in hippocampal neurons using the novel photoconvertible fluorescent protein Dendra2. (ekstern lenke)

Errata

• Hellesvik, Monica; Øye, Hanne; Aksnes, Henriette (2023). Author Correction: Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays (Scientific Reports, (2020), 10, 1, (14680), 10.1038/s41598-020-71538-1). (ekstern lenke)

Poster

• Caiella, Alessia; Hjellvoll, Therese Soltvedt; Arnesen, Thomas et al. (2022). α-Synuclein N-terminal acetylation affects aggregation and membrane binding properties in cell models. (ekstern lenke)
• Hellesvik, Monica; Furnes, Birger Hjønnevåg; Furre, Kristine Haugen et al. (2022). Cellular actin properties depending on NAA80-mediated N-terminal acetylation. (ekstern lenke)
• Chelban, Viorica; Aksnes, Henriette; Pennavaria, Ajia Rae et al. (2022). NAA60 Variants associated with neurological disease have impaired N-terminal acetylation capacity. (ekstern lenke)
• Øye, Hanne; Nundal, Camilla; Aksnes, Henriette et al. (2022). Actin’s N-terminal acetyltransferase NAA80 regulates cytoskeleton morphology. (ekstern lenke)
• Furre, Kristine Haugen; Kianian, Atefeh; Arnesen, Thomas et al. (2020). The Impact of NAA80 and Actin N-terminal Acetylation on Drug Sensitivity. (ekstern lenke)
• Beigl, Tobias B.; Kjosås, Ine; Seljeseth, Emilie et al. (2020). Efficient and crucial quality control of HAP1 cell ploidy status. (ekstern lenke)
• Hellesvik, Monica; Beigl, Tobias B.; Arnesen, Thomas et al. (2020). Spying on the cells using holographic live cell imaging - revealing increased metastatic potential for cancer cells lacking NAA80 . (ekstern lenke)
• Øye, Hanne; Arnesen, Thomas; Aksnes, Henriette (2020). Impact of N‐terminal acetylation on cancer cell invasiveness. (ekstern lenke)
• Beigl, Tobias B.; Glomnes, Nina; Arnesen, Thomas et al. (2019). Knockout of the posttranslational actin modifier NAA80/NatH cause Golgi fragmentation. (ekstern lenke)
• Beigl, Tobias B.; Glomnes, Nina; Arnesen, Thomas et al. (2019). Actin N-terminal acetylation impacts Golgi structural integrity. (ekstern lenke)
• Hellesvik, Monica; Arnesen, Thomas; Aksnes, Henriette (2019). NAA80 KO cells – Fast and Furious? Actin N-terminal acetylation by NatH/NAA80 affects cell adhesion and early cell spreading. (ekstern lenke)
• Aksnes, Henriette; Hellesvik, Monica; Beigl, Tobias B. et al. (2019). Impact of actin N-terminal acetylation. (ekstern lenke)
• Drazic, Adrian; Aksnes, Henriette; Marie, Michael Bruno Eric et al. (2018). Discovery of NAA80 as actin’s N-terminal acetyltransferase – a novel regulator of cytoskeleton dynamics. (ekstern lenke)
• Osberg, Camilla; Aksnes, Henriette; Van Damme, Petra et al. (2018). Saccharomyces cerevisiae growth assays and proteomics reveal functional conservation, redundancy and substrates of N-terminal acetyltransferases. (ekstern lenke)
• Aksnes, Henriette; Drazic, Adrian; Marie, Michaël Bruno Eric et al. (2018). N-terminal acetylation of actin by NAA80 impacts cell migration. (ekstern lenke)
• Aksnes, Henriette; Drazic, Adrian; Marie, Michaël Bruno Eric et al. (2018). Actin Acetylation. (ekstern lenke)
• Varland, Sylvia; Aksnes, Henriette; Jonckheere, Veronique et al. (2016). Dynamic aspects of protein N-terminal acetylation in response to changes in nutrient availability. (ekstern lenke)
• Osberg, Camilla; Ninzima, Sandra; Aksnes, Henriette et al. (2015). Evolutionary conservation of N-terminal acetyltransferase C (NatC) from yeast to human. (ekstern lenke)
• Varland, Sylvia; Van Damme, Petra; Hollebeke, Jolien et al. (2015). Metabolic Regulation of Protein N-terminal Acetylation. (ekstern lenke)
• Aksnes, Henriette; Goris, Marianne; Van Damme, Petra et al. (2015). N-terminal acetylation meets the organelles. (ekstern lenke)
• Osberg, Camilla; Ninzima, Sandra; Aksnes, Henriette et al. (2015). Targeting of membrane proteins by means of N-terminal acetylation. (ekstern lenke)
• Aksnes, Henriette; Hole, Kristine; Van Damme, Petra et al. (2012). ISOLATION OF SECRETED PROTEINS FROM YEAST AND MAMMALIAN CELL CULTURE. (ekstern lenke)
• Starheim, Kristian Kobbenes; Kalvik, Thomas Vikestad; Hole, Kristine et al. (2012). Depletion of the N-terminal acetyltransferase hNaa30p disrupts Golgi integrity. (ekstern lenke)
• Aksnes, Henriette; Osberg, Camilla; Varland, Sylvia et al. (2012). Dynamicity and functional implications of N-terminal acetylation in S. cerevisiae. (ekstern lenke)
• Aksnes, Henriette; Osberg, Camilla; Glomnes, Nina et al. (2012). N-terminal acetyltransferase 30 (Naa30p/NatC) - Phenotypes of S. Cerevisiae naa30Δ strains. (ekstern lenke)
• Varland, Sylvia; Aksnes, Henriette; Damme, Petra Van et al. (2012). GLUCOSE DEPENDENT DYNAMIC N-TERMINAL ACETYLATION IN SACCHAROMYCES CEREVISIAE. (ekstern lenke)
• Aksnes, Henriette; Nair, Rajeevkumar Raveendran; Bramham, Clive R. (2009). Time-lapse imaging of Arc protein degradation and synthesis in hippocampal neurons. (ekstern lenke)

Vitenskapelig foredrag

• Aksnes, Henriette (2022). Protein N-terminal modifications and neurodegenerative disease. (ekstern lenke)
• Aksnes, Henriette (2022). Membrane protein modifications in neurodegenerative disease. (ekstern lenke)
• Hellesvik, Monica; Aksnes, Henriette (2020). Spying on the cells using holographic live cell imaging - revealing increased metastatic potential for cancer cells lacking NAA80 (Talk by Monica Hellesvik selected from Abstracts) . (ekstern lenke)
• Aksnes, Henriette (2020). Efficient and crucial quality control of HAP1 cell ploidy status (Talk selected from abstracts). (ekstern lenke)
• Aksnes, Henriette (2019). Knockout of the posttranslational actin modifier NAA80/NatH cause Golgi fragmentation (Talk selected from abstract). (ekstern lenke)
• Beigl, Tobias B.; Aksnes, Henriette (2019). Actin N-terminal acetylation impacts Golgi structural integrity (Talk by Tobias B. Beigl selected from abstracts). (ekstern lenke)

Short communication

• Morrison, Jennifer; Altuwaijri, Norah; Brønstad, Kirsten Marie et al. (2021). Missense NAA20 variants impairing the NatB protein N-terminal acetyltransferase cause autosomal recessive developmental delay, intellectual disability, and microcephaly. (ekstern lenke)
• Aksnes, Henriette; Marie, Michael Bruno Eric; Arnesen, Thomas (2015). Holding it together: Naa60 at the Golgi. (ekstern lenke)

Faglig foredrag

• Varland, Sylvia; Van Damme, Petra; Hollebeke, Jolien et al. (2015). Metabolic Regulation of N-Terminal Acetylation in Saccharomyces cerevisiae. (ekstern lenke)

Doktorgradsavhandling

• Aksnes, Henriette (2014). N-terminal acetyltransferases NatC and NatF: cellular functions and substrates. (ekstern lenke)

Se en full oversikt over publikasjoner i Cristin

 

FAGFELLEVURDERTE VITENSKAPELIGE PUBLIKASJONER (PubMed)

 

Assessing N-terminal acetylation status of cellular proteins via an antibody specific for acetylated methionine.

Larsen SK, Bekkelund ÅK, Glomnes N, Arnesen T, Aksnes H✉️. 

Biochimie. 2024 Jul 20:S0300-9084(24)00166-4. doi: 10.1016/j.biochi.2024.07.007. Online ahead of print.PMID: 39038730 Free article.

 

Biallelic NAA60 variants with impaired N-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications.

Chelban V✉️, Aksnes H✉️, Maroofian R, LaMonica LC, Seabra L, Siggervåg A, Devic P, Shamseldin HE, Vandrovcova J, Murphy D, Richard AC, Quenez O, Bonnevalle A, Zanetti MN, Kaiyrzhanov R, Salpietro V, Efthymiou S, Schottlaender LV, Morsy H, Scardamaglia A, Tariq A, Pagnamenta AT, Pennavaria A, Krogstad LS, Bekkelund ÅK, Caiella A, Glomnes N, Brønstad KM, Tury S, Moreno De Luca A, Boland-Auge A, Olaso R, Deleuze JF, Anheim M, Cretin B, Vona B, Alajlan F, Abdulwahab F, Battini JL, İpek R, Bauer P, Zifarelli G, Gungor S, Kurul SH, Lochmuller H, Da'as SI, Fakhro KA, Gómez-Pascual A, Botía JA, Wood NW, Horvath R, Ernst AM, Rothman JE, McEntagart M, Crow YJ, Alkuraya FS, Nicolas G; SYNaPS Study Group; Arnesen T✉️, Houlden H✉️.

Nat Commun. 2024 Mar 13;15(1):2269. doi: 10.1038/s41467-024-46354-0.PMID: 38480682 Free PMC article.

 

Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae.

Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T, Olsen JV.

Nat Commun. 2023 Jul 27;14(1):4517. doi: 10.1038/s41467-023-40224-x. PMID: 37500638 Free PMC article.

 

NATs at a glance.

Aksnes H✉️, McTiernan N, Arnesen T✉️.

J Cell Sci. 2023 Jul 15;136(14):jcs260766. doi: 10.1242/jcs.260766. Epub 2023 Jul 18. PMID: 37462250 Review.

 

Protein Termini 2022: central roles of protein ends.

Arnesen T✉️, Aksnes H✉️, Giglione C✉️.

Trends Biochem Sci. 2023 Jun;48(6):495-499. doi: 10.1016/j.tibs.2023.02.008. Epub 2023 Mar 29. PMID: 36997368

 

Actin finally matured – uncovering the machinery and the impact

Arnesen T✉️ and Aksnes H✉️
Trends Biochem Sci 2023 PMID: 36804256
Commentary on: Haahr P et al., Science 2022 10.1126/science.abq5082

 

Expanded in vivo substrate profile of the yeast N-terminal acetyltransferase NatC

Van Damme P✉️, Osberg C S, Jonckheere V, Glomnes N, Gevaert K, Arnesen T and Aksnes H✉️ 
J Biol Chem, 2023 299(2):102824. PMID: 36567016

 

Missense NAA20 variants impairing the NatB protein N-terminal acetyltransferase cause autosomal recessive developmental delay, intellectual disability, and microcephaly.

Morrison J*, Altuwaijri NK*, Brønstad K*, Aksnes H*, Alsaif HS, Evans A, Hashem M, Wheeler PG, Webb BD, Alkuraya FS, Arnesen T.

Genet Med. 2021 Nov;23(11):2213-2218. doi: 10.1038/s41436-021-01264-0. Epub 2021 Jul 6. PMID: 34230638

*Shared first authorship

 

Efficient and crucial quality control of HAP1 cell ploidy status.

Beigl TB, Kjosås I, Seljeseth E, Glomnes N, Aksnes H✉️.

Biol Open. 2020 Nov 12;9(11):bio057174. doi: 10.1242/bio.057174. PMID: 33184093

✉️Corresponding author

 

Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays

Hellesvik M, Øye H, Aksnes H✉️. 

Sci Rep. 2020 Sep 7;10(1):14680. doi: 10.1038/s41598-020-71538-1. PMID: 32895419 

✉️Corresponding author

 

N-terminal acetylation of actin by NAA80 is essential for structural integrity of the Golgi apparatus

Beigl TB, Hellesvik M, Saraste J, Arnesen T, Aksnes H✉️.

Exp Cell Res. 2020 May 15;390(2):111961. doi: 10.1016/j.yexcr.2020.111961. Epub 2020 Mar 21. PMID: 32209306 

✉️Corresponding author

 

Co-translational, Post-translational, and Non-catalytic Roles of N-Terminal Acetyltransferases

Aksnes H✉️, Ree R, Arnesen T*.

Mol Cell. 2019 Mar 21;73(6):1097-1114. Review. PMID: 30878283

✉️Corresponding author

 

Actin polymerization and cell motility are affected by NAA80-mediated posttranslational N-terminal acetylation of actin

Aksnes H✉️, Marie M, Arnesen T, Drazic A.

Commun Integr Biol. 2018 Oct 21;11(4):e1526572. PMID: 30534344

✉️Corresponding author

 

N-terminal Acetylation Levels Are Maintained During Acetyl-CoA Deficiency in Saccharomyces cerevisiae

Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T.

Mol Cell Proteomics. 2018 Dec;17(12):2309-2323. PMID: 30150368

 

NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility

Drazic A^#, Aksnes H^#, Marie M^#, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T.

Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4399-4404. PMID: 29581253

# Shared first authorship

Best paper of the year, Faculty of Medicine, UiB

Received attention:

- From the cover Proc Natl Acad Sci USA 2018; vol. 115 no. 17.

- Commented in: NATure of actin amino-terminal acetylation Proc Natl Acad Sci USA 115(17):4314-16.

- Commented in: Actin's N-terminal acetyltransferase uncovered Cytoskeleton 75(7):318-22.

 

Molecular determinants of the N-terminal acetyltransferase Naa60 anchoring to the Golgi membrane

Aksnes H, Goris M, Strømland Ø, Drazic A, Waheed Q, Reuter N, Arnesen T.

J Biol Chem. 2017 Apr 21;292(16):6821-6837. PMID: 28196861

 

Microscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases

Osberg C, Aksnes H, Ninzima S, Marie M, Arnesen T.

Sci Rep. 2016 Aug 24;6:31627. PMID: 27555049

 

First Things First: Vital Protein Marks by N-Terminal Acetyltransferases

Aksnes H, Drazic A, Marie M, Arnesen T.

Trends Biochem Sci. 2016 Sep;41(9):746-760. Review. PMID: 27498224

 

Holding it together: Naa60 at the Golgi

Aksnes H, Marie M, Arnesen T.

Oncotarget. 2015 Jun 30;6(18):15726-7. PMID: 26164078

 

(Hyper)tension release by N-terminal acetylation

Aksnes H, Drazic A, Arnesen T.

Trends Biochem Sci. 2015 Aug;40(8):422-4. PMID: 26027460

 

Molecular, cellular, and physiological significance of N-terminal acetylation

Aksnes H, Hole K, Arnesen T.

Int Rev Cell Mol Biol. 2015;316:267-305. Review. PMID: 25805127

 

An organellar Nα-acetyltransferase, Naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity

Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, Støve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T.

Cell Rep. 2015 Mar 3;10(8):1362-74. PMID: 25732826

Best paper of the year, Dept. of Molecular Biology, UiB

 

N-terminal acetylation by NatC is not a general determinant for substrate subcellular localization in Saccharomyces cerevisiae.

Aksnes H, Osberg C, Arnesen T.

PLoS One. 2013 Apr 15;8(4):e61012. doi: 10.1371/journal.pone.0061012. Print 2013.

PMID: 23613772 

 

The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4.

Hole K, Van Damme P, Dalva M, Aksnes H, Glomnes N, Varhaug JE, Lillehaug JR, Gevaert K, Arnesen T.

PLoS One. 2011;6(9):e24713. doi: 10.1371/journal.pone.0024713. Epub 2011 Sep 15.

PMID: 21935442 

 

Oversikt over fagfellevurderte publikasjoner i databasen PubMed

Henriette Aksnes - PubMed