Practical Biochemistry and Molecular Biology

Objectives and Content

The course is experiment-oriented and includes selected basic methods in physical biochemistry, biochemical analysis and separation methods, gene technology and microscopy. The course includes exercises in spectrophotometry, chromatography, enzymology, electrophoresis, isolation of biological macromolecules, immunological detection methods and other important methods in gene technology. Work with different biological systems will be emphasised. Organisation of the laboratory work and combining use of different methods for analysis of specific issues will be important. Safety in the laboratory environment and journal writing will also be covered. MOL300 is compulsory for the Master's Programme in Molecular Biology.

Knowledge:

The student

• can explain the theories underlying both basic and some advanced methods in biochemistry and molecular biology
• can explain advantages and disadvantages of the most important methods in biochemistry and molecular biology
• can work with DNA, RNA and proteins
• is familiar with the appropriate laboratory safety regulations both with regard to personal and environmental aspects.

Skills:

The student

• can use a broad range of both basic and some advanced methods in biochemistry and molecular biology (including chromatography, electrophoresis, spectroscopy, enzyme kinetics, molecular cloning, cell culture work and microscopy)
• can examine, assess, interpret and communicate data acquired from laboratory experiments related to biochemistry and molecular biology
• can keep a detailed and accurate laboratory journal

General competence:

The student

• can do experimental work related to biochemistry and molecular biology without detailed instruction and supervision.

Autumn.

This course has a limited capacity, enrolment is based on application. The application deadline is Wednesday in week 33 for the autumn semester. Please see this page for more information. You will receive confirmation of whether you received a seat in Studentweb no later than Monday the week after the deadline.

It is compulsory to attend the first lecture/orientation meeting, or you risk losing your seat. If you are unable to attend the first lecture, you must contact the Study Section (studie.bio@uib.no). The time of the first lecture/orientation meeting can be found in the schedule on the course website or on Mitt UiB.