Basic Course in Flow Cytometry

Postgraduate course

Course description

Objectives and Content

Aim: Basic introduction to flow cytometry. The practical part of the course should enable the students to understand applications for flow cytometry. In addition, the applications used during the practical part of the course will be introduced in seminars by the course leaders.


Theoretical part:

-The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.

Practical part: Hands-on training at one of the local flow cytometers (BD AccuriC6)

- Quantification of apoptotic cells

- Cell cycle analyses

- Simple staining of cells with surface markers

- Analysis of transfected cells

Learning Outcomes

On completion of the course HUIMM307 the student is able to/has:


- understanding for basic applications for flow cytometry

- knowledge on which and how many different fluorochromes one can use on a given flow cytometer

- evaluate the quality of flow cytometry data


- plan and perform basic experiments using flow cytometry

- quality control generated flow cytometry data

- present flow cytometry data in a scientific way


- understanding of basic flow cytometry

- understanding as to why to use flow cytometry in a certain research question

- interpret basic flow cytometry data

ECTS Credits


Level of Study

Master students, PhD candidates

Semester of Instruction


There is no direct registration in this course, but each person that would like to attend has to contact Brith Bergum as there is a limited number of spaces.

Place of Instruction

Core facility for flow cytometry, Department of Clinical Science, Laboratory building 5th floor
Required Previous Knowledge

Basic knowledge on apoptosis and cell cycle.

Experience with basic laboratory techniques such as pipetting and centrifugation.

Recommended Previous Knowledge
Biology or molecular biology on bachelor level.
Access to the Course

The course will only be given if at least 4 students register for the course, and is limited to 10 students.

Course-coordinator decides who can participate.

Teaching and learning methods
23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work
Compulsory Assignments and Attendance
Compulsory participation in all experiments.
Forms of Assessment
Oral presentation of results and written lab report (composed of Introduction, Materials and Methods, Results, Discussion). Both have to be approved by the course leader.
Grading Scale
Assessment Semester


Because of lack of necessary equipment, the course must unfortunately be cancelled for Autumn 2021. We hope to be able to arrange the course Spring 2022.

Reading List

Flow cytometry - A Basic Introduction,

Wiki version; Michael Ormerod.

Course Evaluation
After every course
Examination Support Material
Course Administrator
Thea Midtun (

Bergen Research School in Inflammation, Broegelmann Research Laboratory,

Department of Clinical Science.