Model Systems
To understand the molecular mechanisms driving brain metastases (BM) and to develop new treatment strategies, the use of relevant animal model systems is essential.
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Xenograft models
We have developed novel xenograft models (external link) in our laboratory, where human BM tumor cell lines from melanoma, lung cancer, and breast cancer are introduced into the bloodstream of immunodeficient nod/scid mice. Using a highly standardized protocol, the cells are injected into the left cardiac ventricle, and tumor development is monitored with advanced multimodal molecular imaging techniques. Within 3 to 6 weeks, all animals consistently develop tumors that show a marked preference for the brain. MR imaging reveals multiple BM, closely resembling the patterns observed in patients. These models are among the very few that can systematically and reproducibly mimic the metastatic spread of cancer to the brain as it occurs in clinical settings.
Patient biobank
In parallel, we utilize an ethically approved patient biobank in which BM tissue and blood samples are continuously collected and stored. This valuable resource allows us to study whether specific genetic mutations contribute to the metastatic spread of cancer to the brain. Through these analyses, we aim to expand our molecular understanding of the disease, which in turn can inform the design of new targeted treatment strategies for patients with BM.