System spesifications
Lasers
405 (100 mW), 488 (150 mW), 561 (100 mW) and 637 nm (140 mW).
Objectives
M/NA= magnification/numerical aperture WD= working distance in mm
- 10x / 0.45 CFI Plan Apochromat Lambda Air WD 4.0
- 20x / 0.75 CFI Plan Apochromat Lambda Air WD 1.0
- 40x / 1.14 CFI Plan Apochromat Lambda S LWD Water WD 0.59
- 60X / 1.40 CFI Plan Apochromat Lambda Oil WD 0.14
- 100x / 1.49 CFI SR HP Apo TIRF Oil WD 0.12
Detectors
Camera based system:
- iXon 888 Life EMCCD (1024x1024)
- Zyla 4.2 PLUS sCMOS (2048x2048)
Live cell imaging
- In general very well suited for live cell experiments.
- OkoLab large heating incubator with CO2 control.
- Perfect focus system (PFS)
- Possible to set up multi-positions over time.
TIRF
Total Internal Reflection Fluorescence uses an evanescent wave from total internal reflection to excite fluorescence only within ~100–200 nm of a surface. This enables high-contrast imaging of processes at cell membranes.
3D tile scans
Can scan and stitch individual z-stack over large fields of interest at any magnification.
More efficient system for scanning larger samples in depth compared to a point scanner confocals.