System spesifications
Lasers
- Blue diode (BD) - 50 mW 405 nm
- White Laser (WL) - lambda lange 470-670 nm, freely tunable in 1 nm steps, power approx. 1.5 mW per line, pulsed
Detectors
Five detectors: 2 PMTs and 3 HyDs (the two first HyDs are are degraded due to extensive use)
Objectives
20x/0.75 HC PL APO CS2 IMM (Water, Glyc, Oil) WD 0.68
25x/0.95 HC Fluotar VISIR Water WD 2.40
40x/1.1 HC PL APO motCORR CS2 Water WD 0.65
93x/1.3 HC PL APO motCORR STED WHITE Glycerin WD 0.30
100x/1.4 HC PL APO STED WHITE Oil WD 0.13
Software modules
- Lightning - Lightning enables high resolution imaging and image enhancement by adaptive deconvolution.
- Navigator - for multi-positions and tile-scan imaging
- FRET - Fluorescence Recovery After Photobleaching
- FRAP - Förster Resonance Energy Transfer
Live Cell imaging
- Resonant scanner for fast live cell imgaing with lower light exposure.
- Tokai Hit Stage Inkubatorsystem for temperature (370C) and CO2-controll.
- Auto Focus Control (AFC).