Nikon Eclipse Ti2 spinning disk confocal/Widefield/TIRF/SMLM microscope

For widefield fluorescens and CrestOptics X-Light V3 spinning disk confocal: Lumencor Celesta Quattro, 4 channels; 405 nm, 477 nm, 546 nm and 640 nm 

For TIRF and activation/bleaching experiments: LUN-F laser unit with 405 nm (70 nW), 488 nm (70 mW), 561 nm(60 mW) and 640 nm (50 mW) lasers.

NA= numerical aperture WD= working distance in mm

• 10x Air Plan Fluor NA0,30 WD 15,2 Phase contrast (Ph1)
• 20x Air Plan Fluor (coverslip correction) NA0,45 WD 7,4 Phase contrast (Ph1)
• 20x Air Plan Apochromat lambda NA0,75 WD 1,0 Fluorescence
• 40x Air Plan Apochromat lambda (coverslip correction) NA0,95 WD 0,21 Phase contrast (Ph2) / fluorescence
• 60x Oil CFI Apo TIRF (coverslip and temperature correction) NA1,49 WD 0,12 TIRF / fluorescence
• 100x Oil CFI Plan Apochromat lambda NA1,45 WD 0,13 Fluorescence

Camera based system: 

• For any fluorescence detection: Monochrome Prime BSI Express Scientific CMOS 
• For brightfield color staining: DS-Fi3 CMOS.

• In general very well suited for live cell timelapse (fast, long term, multi-positions).
• OkoLab stage top heating incubator with CO² control.
• Perfect focus system (PFS).
• Possible to set up multi-positions over time. 
• Bleaching (FRAP) and activation (PA) experiments.  

Total Internal Reflection Fluorescence uses an evanescent wave from total internal reflection to excite fluorescence only within ~100–200 nm of a surface. This enables high-contrast imaging of processes at cell membranes.

Single-Molecule Localization Microscopy is a super-resolution technique that localizes individual fluorescent molecules to reconstruct images beyond the diffraction limit. This system is rigged to accommodate the following applications:

• Single partical tracking (spt-PALM)
• PALM (Photo Activated Localization Microscopy)
• DNA-Paint 

Support on these applications are handled through NORBRAIN, not MIC.