Olympus Multiphoton FVMP-RS

Olympus Multiphoton FVMP-RS was installed in 2020 and funded through the NRC NORMOLIM infrastructure. Multiphoton allows imaging deeper into tissue than confocal microscopy. However, the achievable imaging depth depends strongly on the tissue’s structure and optical properties, such as scattering and transparency, so penetration depth can vary considerably. For fixed samples, tissue clearing methods can be used to improve transparency and extend imaging depth.

System spesifications

Laser

Mai Tai DeepSee HP (Spectra Physics) is an Ultrafast Ti:Sapphire Laser with Automated Dispersion Compensation and tuning range of 690-1040 nm. The laser is specifically tailored to the FVMPR-RS system.

Objective

25x NA1.05 Olympus (XPLN25XWMP2) with motorized correction collar and 2 mm working distance. Max field of view 505x505 um. Dip-in water immersion.

Detectors

2 non-descanned GaAsp-detectors (NDD).

Allows simultaneous two-color imaging with the possible color combinations: Blue (455-490nm)/Green-Yellow (515-560) emission or Green (495-540nm)/Red (575-645) emission.

Scanner

FVMPE-RS hybrid scan unit with standard point scanning and resonant galvanometer scanners.

Typical samples

Thick fixed samples.
3D-cell culture models.
Ex vivo models; explant tissue, organoids, spheroids.

Responsible personnel and location

Emails: hege.dale@uib.no; emmet.mc.cormack@uib.no

Mazemap directions to Olympus Multiphoton

Last updated: 04.06.2026