Olympus Slide-scanner microscope

Olympus VS120 S6 Slide scanner was installed 2018 and funded by the Faculty of Medicine. It is dedicated for scanning microscopy slides of tissue preparations stained with histochemistry (HC), immunohistochemistry (IHC) or immunofluorescence (IF). Six slides can be mounted on the stage at the time.

System spesifications

Detectors

Camera based system:

For IF: Hamamatsu ORCA-Flash 4.0 V3 B/W camera (2048x2048 px, 6.5 um²)
For HC and ICH: Allied Vision Pike F-505 color camera (2452v2054 px, 3.45 um²)

Objectives

M/NA= magnification/ numerical aperture, all AIR objectives. Px= pixel size in um

2x/0.08 PlanApoN Px: 3.3
4x/0.16 UPlanSApo Px: 1.65
10x/0.4 UPlanSApo Px: 0.66
20x/0.75 UPlanSApo Px: 0.33
40x/0.95 UPlanSApo Px: 0.17

Fluorescence

LED-lamp light source.

Quad-band filter with the following excitation (ex) and emission (em) bandpasses:

Blue: ex 340-380, em 430-480
Green: ex 480-500, em 507-543
Red: ex 543-586, em 579-631
Far red: ex 630-660, em 669-741

Viewing and analysis

QuPath: A powerful freeware that opens VSI-files (raw format from the slide scanner). It is compatible with Windows, Mac OS X and Linux. This is a software under continous development, so make sure that you occasionally check for updates.

Installation and information Welcome to QuPath!
System requirements.
Tutorials on YouTube recommended for first time users.
Webinar "Quantitative Pathology & BioImage Analysis: QuPath" 29 April, 2020.
Important: Remember to cite the software in your work!

Responsible personnel and location

Emails: hege.dale@uib.no; endy.spriet@uib.no

Mazemap directions to Olympus slide scanner

Last updated: 02.06.2026