Se vår engelsk nettside: Inari Kursula lab

Methods in Biomedical Research - BMED320

2024

• Khorsand, Fahimeh; Haug, Bengt Erik; Kursula, Inari Talvikki et al. (2024). Expression and purification of human neutrophil proteinase 3 from insect cells and characterization of ligand binding. (ekstern lenke)

2023

• Dans, Madeline G.; Piirainen, Henni; Nguyen, William et al. (2023). Sulfonylpiperazine compounds prevent Plasmodium falciparum invasion of red blood cells through interference with actin-1/profilin dynamics. (ekstern lenke)
• Lopez Moreno, Andrea Johana; Andreadaki, Maria; Vahokoski, Juha et al. (2023). Structure and function of Plasmodium actin II in the parasite mosquito stages. (ekstern lenke)
• Trones, Maiken Fridal; Kursula, Inari Talvikki (2023). Structural and biochemical characterization of ATP hydrolysis and phosphate release in malaria parasite actin II mutants. (ekstern lenke)

2022

• Bendes, Ábris Ádám; Kursula, Petri; Kursula, Inari (2022). Structure and function of an atypical homodimeric actin capping protein from the malaria parasite. (ekstern lenke)
• Vahokoski, Juha; Calder, Lesley J.; Moreno, Andrea Johana Lopez et al. (2022). High-resolution structures of malaria parasite actomyosin and actin filaments. (ekstern lenke)
• Pires, Isa; Hung, Yu-Fu; Bergmann, Ulrich et al. (2022). Analysis of Plasmodium falciparum myosin B ATPase activity and structure in complex with the calmodulin-like domain of its light chain MLC-B. (ekstern lenke)

2021

• Bustad, Helene J.; Kallio, Juha Pekka; Laitaoja, Mikko et al. (2021). Characterization of porphobilinogen deaminase mutants reveals that arginine-173 is crucial for polypyrrole elongation mechanism. (ekstern lenke)

2020

• Makumire, Stanley; Zininga, Tawanda; Vahokoski, Juha et al. (2020). Biophysical analysis of Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop) reveals a monomer that is characterised by folded segments connected by flexible linkers. (ekstern lenke)
• Bendes, Ábris Ádám; Chatterjee, Moon; Götte, Benjamin et al. (2020). Functional homo- and heterodimeric actin capping proteins from the malaria parasite. (ekstern lenke)
• Moreau, Catherine A.; Quadt, Katharina A.; Piirainen, Henni et al. (2020). A function of profilin in force generation during malaria parasite motility that is independent of actin binding. (ekstern lenke)

2019

• Saghaug, Christina Skår; Klotz, Christian; Kallio, Juha Pekka et al. (2019). Genetic variation in metronidazole metabolism and oxidative stress pathways in clinical giardia lamblia assemblage A and B isolates. (ekstern lenke)
• Kumpula, Esa-Pekka; Lopez Moreno, Andrea Johana; Tajedin, Leila et al. (2019). Atomic view into plasmodium actin polymerization, ATP hydrolysis, and fragmentation. (ekstern lenke)

2018

• Szigetvari, Peter Daniel; Muruganandam, Gopinath; Kallio, Juha et al. (2018). The quaternary structure of human tyrosine hydroxylase: effects of dystonia‐associated missense variants on oligomeric state and enzyme activity. (ekstern lenke)
• Mukherjee, Budhaditya; Tessaro, Francesca; Vahokoski, Juha et al. (2018). Modeling and resistant alleles explain the selectivity of antimalarial compound 49c towards apicomplexan aspartyl proteases. (ekstern lenke)

2017

• Pospich, Sabrina; Kumpula, Esa-Pekka; Von Der Ecken, Julian et al. (2017). Near-atomic structure of jasplakinolide-stabilized malaria parasite F-actin reveals the structural basis of filament instability. (ekstern lenke)
• Muruganandam, Gopinath; Raasakka, Arne; Myllykoski, Matti et al. (2017). Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase. (ekstern lenke)
• Pino, Paco; Caldelari, Reto; Mukerhjee, Budhaditya et al. (2017). A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress. (ekstern lenke)
• Kumpula, Esa-Pekka; Pires, Isa; Lasiwa, Davki et al. (2017). Apicomplexan actin polymerization depends on nucleation. (ekstern lenke)
• Panneerselvam, Saravanan; Kumpula, Esa-Pekka; Kursula, Inari et al. (2017). Rapid cadmium SAD phasing at the standard wavelength (1 Å). (ekstern lenke)
• Green, Judith L.; Wall, Richard J. Wall; Vahokoski, Juha et al. (2017). Compositional and expression analyses of the glideosome during the Plasmodium life cycle reveal an additional myosin light chain required for maximum motility. (ekstern lenke)
• Moreau, Catherine A.; Bhargav, Saligram P.; Kumar, Hirdesh et al. (2017). A unique profilin-actin interface is important for malaria parasite motility. (ekstern lenke)

2016

• Müller, Christina; Samoo, Atta; Hammoudi, Pierre-Mehdi et al. (2016). Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein. (ekstern lenke)
• Jacot, Damien; Tosetti, Nicolò; Pires, Isa et al. (2016). An apicomplexan actin-binding protein serves as a connector and lipid sensor to coordinate motility and invasion. (ekstern lenke)

2015

• Bhargav, Saligram Prabhakar; Vahokoski, Juha; Kallio, Juha Pekka et al. (2015). Two independently folding units of Plasmodium profilin suggest evolution via gene fusion. (ekstern lenke)
• Raasakka, Arne; Myllykoski, Matti; Laulumaa, Saara et al. (2015). Determinants of ligand binding and catalytic activity in the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase. (ekstern lenke)

Se en full oversikt over publikasjoner i Cristin

2022

• Pires I, Hung YF, Bergmann U, Molloy JE & Kursula I (2022) Characterization of Plasmodium falciparum myosin B in complex with the calmodulin-like domain of its light chain MLC-B. Submitted, preprint available on bioRxiv: https://biorxiv.org/cgi/content/short/2022.05.28.493848v1

• Vahokoski J, Calder L, Lopez AJ, Molloy JE, Kursula I & Rosenthal PB (2022) High-resolution structures of malaria parasite actomyosin and actin filaments. PLoS Pathog 18: e1010408.

• Bendes ÁÁ, Kursula P & Kursula I (2022) Structure and function of an atypical homodimeric actin capping protein from the malaria parasite. Cell Mol Life Sci 79: 125.

2021

• Han H, Round E, Schubert R, Gül Y, Makroczyová J, Meza D, Heuser P, Aepfelbacher M, Barák I, Betzel C, Fromme P, Kursula I, Nissen P, Tereschenko E, Schulz J, Uetrecht C, Ulicný J, Wilmanns M, Hajdu J, Lamzin VS & Lorenzen K. (2021) The XBI BioLab for life science experiments at the European XFEL. J Appl Crystallogr 54: 7-21.

• Bustad HJ, Kallio JP, Laitaoja M, Toska K, Kursula I, Martinez A & Jänis J (2021) Characterization of porphobilinogen deaminase mutants reveals that arginine-173 is crucial for polypyrrole elongation mechanism. iScience 24: 102152.

2020

• Makumire S, Zininga T, Vahokoski J, Kursula I & Shonhai A (2020) Biophysical analysis of Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop) reveals a monomer that is characterised by folded segments connected by flexible linkers. PLoS ONE 15: 0226657.

• Bendes ÁÁ, Chatterjee M, Götte B, Kursula P & Kursula I (2020) Functional homo- and heterodimeric actin capping proteins from the malaria parasite. Biochem Biophys Res Commun 525: 681-686.

• Moreau CA, Quadt KA, Piirainen H, Kumar H, Bhargav SP, Strauss L, Tolja NH, Wade RC, Spatz JP, Kursula I & Frischknecht F (2020) Optical tweezers uncover a function of profilin in force generation during malaria parasite motility independent of actin binding. J Cell Sci 134: jcs233775.

• Kumpula EP, Lopez AJ, Tajedin L, Han H & Kursula I (2020) Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation. PLoS Biol 17: e3000315.

2019

• Saghaug CS, Klotz C, Kallio JP, Brattbakk HR, Stokowy T, Aebischer T, Kursula I, Langeland N & Hanevik K (2019) Genetic variation in metronidazole metabolism and oxidative stress pathways in clinical Giardia lamblia assemblage A and B isolates. Infect Drug Resist 12: 1221-1235.

• Szigetvari PD, Muruganandam G, Kallio JP, Hallin EI, Fossbakk A, Loris R, Kursula I, Møller LB, Knappskog PM, Kursula P & Haavik J (2019) The quaternary structure of human tyrosine hydroxylase: effects of dystonia-associated missense variants on oligomeric state and enzyme activity. J Neurochem 148: 291-306.

2018

• Mukherjee B, Tessaro F, Vahokoski J, Kursula I, Marq JB, Scapozza L & Soldati-Favre D (2018) Modeling and resistant alleles explain the selectivity of antimalarial compound 49c towards apicomplexan aspartyl proteases. EMBO J 37: e98047.

2017

• Pino P, Caldelari R, Mukherjee B, Vahokoski J, Klages N, Maco B, Collins CR, Blackman MJ, Kursula I, Heussler V, Brochet M & Soldati-Favre D (2017) A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress. Science 358: 522-528.

• Kumpula EP, Pires IP, Lasiwa D, Piirainen H, Bergmann U, Vahokoski J & Kursula I (2017) Apicomplexan actin polymerization depends on nucleation. Sci Rep 7: 12137.

• Pospich S, Kumpula EP, von der Ecken J, Vahokoski J, Kursula I & Raunser S (2017) Near-atomic structure of jasplakinolide-stabilized malaria parasite F-actin reveals the structural basis of filament instability. Proc Natl Acad Sci 114: 10636-10641.

• Green JL, Wall RJ, Vahokoski J, Yusuf NA, Ridzuan MAM, Stanway RR, Stock J, Knuepfer E, Brady D, Martin SR, Howell SA, Pires IP, Moon RW, Molloy JE, Kursula I, Tewari R & Holder AA (2017) Compositional and expression analyses of the glideosome during the Plasmodium life cycle reveal an additional myosin light chain required for maximum motility. J Biol Chem 292: 17857-17875.

• Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A & Meents A (2017) Rapid cadmium SAD phasing at the standard wavelength (1 Å). Acta Cryst D 73: 581-590.

• Moreau C, Bhargav SP, Kumar H, Quadt KA, Piirainen H, Strauss L, Kehrer J, Streichfuss M, Spatz J, Wade R, Kursula I & Frischknecht F (2017) A unique profilin-actin interface is important for malaria parasite motility. PLoS Pathog 13: e1006412.

• Muruganandam G, Raasakka A, Myllykoski M, Kursula I & Kursula P (2017) Structural similarities and functional differences point to evolutionary relationship between tRNA healing enzymes and the myelin enzyme CNPase. BMC Biochem 18: 7.

2016

• Jacot D, Tosetti N, Pires I, Stock J, Graindorge A, Hung YF, Han H, Tewari R, Kursula I & Soldati-Favre D (2016) An apicomplexan actin-binding protein serves as a connector and lipid sensor to coordinate motility and invasion. Cell Host Microbe 20: 731-743.

• Mueller C, Samoo A, Hammoudi PM, Klages N, Kallio JP, Kursula I & Soldati-Favre D (2016) Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein. J Cell Sci 129: 1031-1045.

2015

• Raasakka A, Myllykoski M, Laulumaa S, Lehtimäki M, Haertlein M, Moulin M, Kursula I & Kursula P (2015) Determinants of ligand binding and catalytic activity in the myelin enzyme 2',3-cyclic nucleotide 3'-phosphodiesterase. Sci Rep 5: 16520.

• Bhargav SP, Vahokoski J, Kallio JP, Torda AE, Kursula P & Kursula I (2015) Two independently folding units of Plasmodium profilin suggest evolution via gene fusion. Cell Mol Life Sci 72: 4193-4203.

• Kumpula EP & Kursula I (2015) Towards a molecular understanding of the apicomplexan actin motor – on a road to novel targets for malaria remedies? Acta Cryst F, 71: 500-513.

2014

• Salamun J, Kallio JP, Daher W, Soldati-Favre D & Kursula I (2014) Structure of Toxoplasma gondii coronin – an actin-binding protein that relocalizes to the posterior pole of invasive parasites and contributes to invasion and egress. FASEB J, 28: 4729-4747.

• Vahokoski J, Bhargav SP, Desfosses A, Andreadaki M, Kumpula EP, Muñico Martinez S, Ignatev A, Lepper S, Frischknecht F, Sidén-Kiamos I, Sachse C & Kursula I (2014) Structural differences explain diverse functions of Plasmodium actins. PLoS Pathog, 10: e1004091.

• Kallio JP & Kursula I (2014) Recombinant production, purification, and crystallization of the Toxoplasma gondii coronin WD40 domain. Acta Cryst F, 70: 517-521.

• Ruskamo S, Yadav RP, Sharma S, Lehtimäki M, Laulumaa S, Aggarwal S, Simons M, Bürck J, Ulrich AS, Juffer AH, Kursula I & Kursula P (2014) Atomic-resolution view into structure-function relationships of human myelin peripheral membrane protein P2. Acta Cryst D, 70: 165-176.

2013

• Muruganandam G, Bürck J, Ulrich AS, Kursula I & Kursula P (2013) Lipid membrane association of myelin proteins and peptide segments studied by oriented and synchrotron radiation circular dichroism spectroscopy. J Phys Chem B, 117: 14983-14993.

• Vervaet N, Kallio JP, Meier S, Salmivaara E, Eberhardt M, Zhang S, Sun X, Wu Z, Kursula P & Kursula I (2013) Recombinant production, crystallization, and preliminary structural characterization of Schistosoma japonicum profilin. Acta Cryst F, 69: 1264-1267.

• Wedderhoff I, Kursula I, Groves MR & Ortiz de Orué Lucana D (2013) Iron binding at specific sites within the octameric HbpS protects streptomycetes from iron-mediated oxidative stress. PLoS ONE, 8: e71579.

• Myllykoski M, Raasakka A, Lehtimäki M, Han H, Kursula I & Kursula P (2013) Crystallographic analysis of the reaction cycle of 2',3'-cyclic nucleotide 3'-phosphodiesterase, a member of the 2H phosphoesterase family. J Mol Biol, 425: 4307-4322.

2012

• Ruskamo S, Chukhlieb M, Vahokoski J, Bhargav SP, Liang F, Kursula I & Kursula P (2012) Juxtanodin is an intrinsically disordered F-actin binding protein. Sci Rep 2: 899.

• Myllykoski M, Itoh K, Kangas SM, Heape AM, Kang SU, Lubec G, Kursula I & Kursula P (2012) Direct molecular interaction of the myelin enzyme 2’,3’-cyclic nucleotide 3’-phosphodiesterase with the calcium sensor calmodulin. J Neurochem 123: 515-524.

• Ignatev A, Bhargav SP, Vahokoski J, Kursula P & Kursula I (2012) The lasso segment is required for functional dimerization of the Plasmodium formin 1 FH2 domain. PLoS ONE 7: e33586.

2011

• Singh BK, Sattler JM, Chatterjee M, Huttu J, Schüler H & Kursula I (2011) Crystal structures explain functional differences in the two actin depolymerization factors of the malaria parasite. J Biol Chem 286: 28256-28264.

• Nilsson S, Moll K, Angeletti D, Albrecht L, Kursula I, Jiang N, Sun X, Berzins K, Wahlgren M & Chen Q (2011) Characterization of the Duffy-binding like domain of Plasmodium falciparum blood-stage antigen 332. Malaria Res Treat 2011: 671439.

• Patel AK, Yadav RP, Majava V, Kursula I & Kursula P (2011) Structure of the pro-apoptotic protein kinase DAPK2, a member of the death-associated protein kinase family J Mol Biol 409: 369-383.

2010

• Huttu J, Singh BK, Bhargav SP, Sattler JM, Schüler H & Kursula I (2010) Crystallization and preliminary structural characterization of the two actin depolymerization factors of the malaria parasite. Acta Cryst F 66: 583-587.

• Majava V, Polverini E, Mazzini A, Nanekar R, Knoll W, Peters J, Natali F, Baumgärtel P, Kursula I & Kursula P (2010) Structural and functional characterization of human peripheral nervous system myelin protein P2. PLoS ONE 5: e10300.

• Wigren E, Bourhis JM, Kursula I, Guy JE & Lindqvist Y (2010) Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of Factor V and Factor VIII. FEBS Lett 584: 878-882.

2008

• Kursula I, Kursula P, Ganter M, Panjikar S, Matuschewski K & Schüler H (2008) Structural basis for parasite‐specific functions of the divergent profilin of Plasmodium falciparum. Structure 16: 1638-1648.

• Kursula P, Kursula I, Massimi M, Song YH, Downer, J, Stanley WA, Witke W & Wilmanns M (2008) High‐resolution structural analysis of mammalian profilin 2a complex formation with two physiological ligands: formin homology 1 domain of mDia1 and the proline rich domain of VASP. J Mol Biol 375: 270‐290.

2007

• Sultana A, Alexeev I, Kursula I, Mäntsälä P, Niemi J & Schneider G (2007) Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidase: indications of multidomain pseudomerohedral twinning. Acta Cryst D 63: 149‐159.

2006

• Müller S, Kursula I, Zou P & Wilmanns M (2006) Crystal structure of the PB1 domain of NBR1. FEBS Lett 581: 341‐344.

2005

• Kursula I, Heape AM & Kursula P (2005) Crystal structure of non‐fused glutathione‐S-transferase from Schistosoma japonicum in complex with glutathione. Protein Pept Lett 12: 709‐712.

2004

• Kursula I, Salin M, Sun J, Norledge BV, Haapalainen AM, Sampson NS & Wierenga RK (2004) Understanding protein lids: Structural analysis of active hinge mutants in triosephosphate isomerase. Protein Eng Des Sel 17: 375‐382.

2003

• Kursula I & Wierenga RK (2003) Crystal structure of triosephosphate isomerase complexed with 2‐phosphoglycolate at 0.83‐Å resolution. J Biol Chem 278: 9544‐9551.

2002

• Kursula I, Partanen S, Lambeir AM & Wierenga RK (2002) The importance of the conserved Arg191‐Asp227 salt bridge of triosephosphate isomerase for folding, stability, and catalysis. FEBS Lett 518: 39‐42.

2001

• Kursula I, Partanen S, Lambeir AM, Antonov DM, Augustyns K & Wierenga RK (2001) Structural determinants for ligand binding and catalysis of triosephosphate isomerase. Eur J Biochem 268: 5189‐5196.

2000

• Lambeir AM, Backmann J, Ruiz‐Sanz J, Filimonov V, Nielsen JE, Kursula I, Norledge BV & Wierenga RK (2000) The ionisation of Glu65 buried at the monomer‐monomer interface in Leishmana mexicana triosephosphate isomerase is thermodynamically linked to the stability of the dimer. Eur J Biochem 267: 2516‐2524.

1999

• Pospiech H, Kursula I, Abdel‐Aziz W, Malkas L, Uitto L, Kastelli M, Vihinen‐Ranta M, Eskelinen S & Syväoja JE (1999) A neutralizing antibody against human DNA polymerase ε inhibits cellular but not SV40 DNA replication. Nucleic Acids Res 27: 3799‐3804.