Label-free: Impedance based biosensor technology does not require markers or dyes. Interference-free from Nanomaterials and Nanoparticles perturbation.
xCELLigence system (Agylent Technologies, Inc., USA)
Real-time kinetic readouts: Obtains data continuously from seconds to days. Simultaneously monitor up to three plates, without scheduling conflicts. Easy workflow: Plate cells, expose to Nanomaterials and begin monitoring.
Measures cell number, size, morphology, and attachment properties, with the ability to perform kinetic analysis of cell invasion/migration (CIM).
AmphaZ30 (Amphasys AG, Lucerne, Switzerland)
Amphaz30 is an impedance flow cytometer for high-throughput single-cell characterization without optical components. Single cells pass through a micrometer-sized channel in a chip equipped with microelectrodes. The electrical impedance changes when a cell passes through the applied alternating current (AC) field, permitting cell detection and impedance measurement. The measured impedance is used to assess cellular size, membrane capacitance, and cytoplasm resistance and to differentiate between live and cell cells and modes of cell death.
Cyclic voltammetry for oxidative stress testing
We use cyclic voltammetry (CV) to study if nanomaterials may cause oxidative stress in biological systems. The total antioxidant capacity (TAC) of a biofluid reflects the amount of antioxidants available to counteract oxidative stress.
Module for measurement of transepithelial/endothelial electrical resistance (TEER)
Top, TEER module placed on top of the lung-on-a-chip and hook up to the wires at the microscope stage. Bottom, magnification showing the gold-plated electrodes that are immersed in the cell culture medium at the inlets/outlets of each cell culture chamber of the lung-on-a-chip
Researcher: Ivan Rios-Mondragón, PhD, PI: Mihaela Roxana Cimpan, Professor