Protein production
On DNA level:
- ”Seam-less” cloning methods
- Gateway cloning methods
- Site directed mutagenesis (PCR-based)
- In vitro transcription
- SNP genotyping and sequencing (collaboration with Center of Medical genetics and Molecular biology)
On Protein level:
- Expression and purification of recombinant proteins using E. coli, Pichia pastoris, mammalian cells, and Spodoptera frugiperda
- In vitro translation
- Denaturing and non-denaturing polyacrylamide gel electrophoresis (SDS- and native-PAGE)
- In vitro RNA-protein interactions
- In vitro protein-protein interactions
- Various pull-down interaction assays
- Phosphorylation studies (including MS, carried out by PROBE)
- Immunostaining
- Functional assays (activity, stability, interactions etc)
Cell culture
- Expression of recombinant proteins in different cell lines such as HEK293, HELA, SH-SY5Y
- Stimulation, inhibition and stability assays
- Confocal and fluorescence microscopy
- Functional assays (activity and interactions etc)
Biophysical Methods
- Circular dichroism spectropolarimetry (CD)
- Synchrotron radiation circular dichroism (SRCD)
- Oriented circular dichroism (OCD)
- Surface plasmon resonance (SPR)
- Isothermal titration calorimetry (ITC)
- Differential scanning calorimetry (DSC)
- Atomic force microscopy (AFM)
- Fluorimetric assays, like intrinsic Trp fluorescence
- Turbidimetric assays
- UV-VIS absorption assays
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS, collaboration in Oulu)
- Dynamic light scattering (DLS)
- Thermal shift assays/Differential scanning fluorimetry (aka Thermofluor or DSF)
- Various neutron methods (diffraction, reflectometry, small-angle scattering, EINS, QENS, etc.)
- Transmission electron microscopy (negative stained samples, immunostained samples)
- Static light scattering (SLS/SEC-MALS)
Structural Biology
- Macromolecular X-ray crystallography
- Nuclear magnetic resonance spectroscopy (NMR, collaboration)
- Electron cryomicroscopy (Collaboration)
- Solution small-angle X-ray scattering and diffraction (SAXS/SAXD)
- Molecular modelling and simulation
Magnetic Resonance Imaging
- T1-w/T2-w MRI
- Diffusion Weighted Imaging
- functional Magnetic Resonance Imaging
- Magnetic Resonance Spectroscopy
Animal work
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