High Throughput Sequencing Services
The UiB Genomic Core Facility (GCF) offers a range of different NGS applications. The main volume of samples sequenced at GCF are mRNA-seq and Exome-seq. We sequence a range of different species, from whale, plants, fish to human samples. Our services are used in many research environments, from marine research, basic biology and biomedicine.
High Throughput Sequencing Services
Sequencing systems available through the UiB Genomic Core Facility (GCF):
- Illumina NovaSeqX
- Illumina NovaSeq 6000
- Illumina NextSeq 500
- Illumina MiSeq
NGS applications offered at UiB Genomic Core Facility
End-to-end solutions (from DNA/RNA library preparation, QC, sequencing and bioinformatics):
- Whole genome sequencing
- Exome sequencing
- Whole transcriptome sequencing (totRNA) for SNP detection, expression analysis, and splice variant detection
- mRNA sequencing
Sequencing service:
- 10X single cell sequencing
- Protein-DNA/RNA interactions (eg. ChIP-Seq etc.)
- Targeted sequencing
- Metagenomics
- Sequencing of ready-made libraries from users (quality checked by GCF)
We can perform both single- or paired-end sequencing up to 300bp read lengths
Guidelines
We recommend contacting us at an early stage to schedule a project discussion meeting. Ideally, the Genomics Core Facility and the user should meet before any experimental work begins to minimize common design pitfalls, particularly in gene expression studies.
Quantification and quality control of DNA
Reliable quantification and assessment of DNA integrity are essential for experimental success. High-quality DNA should consist of intact, high-molecular-weight fragments and be free from contaminants such as proteins, carbohydrates, and residual organic solvents.
Quantification and quality control of RNA
Reliable quantification and assessment of RNA integrity are essential for experimental success. The RNA should be intact, without signs of degradation, and free from contaminants such as proteins, carbohydrates, and residual organic solvents.
All submitted RNA samples will undergo quality control using the Agilent TapeStation prior to downstream processing. RNA integrity will be evaluated based on the electropherogram profile and the RNA Integrity Number (RIN). Samples with a RIN below 8.0 are considered suboptimal in quality, and users should be aware of the potential for bias in subsequent analyses.
Sample requirements for library preparation
At the Genomics Core Facility, we offer a range of library preparation protocols. Sample requirements vary depending on the selected protocol; please refer to the table below for details on sample type, input amounts, and quality specifications.
* Some protocols are sensitive to EDTA, contact GCF if you are in doubt.
** Make sure the primers in your PCR are placed >50bp upstream/downstream from your target of interest.
*** This protocol requires input DNA to be larger than 300bp, and thus does not support FFPE, degraded DNA, or small amplicons.